دراسة الظروف المثلى لإنتاج البروتيز المتعادل من العزلة المحلية Aspergillus niger var. carbonarius

الملخص EN

The optimum conditions for the production of neutral protease from local strain Aspergillus niger var carbonarius by solid - state fermentation system (Wheat bran) moisted with 0.2 M phosphate buffer (PH7.0) .

the hydration ratio was 1:5 (V:W) .

the concentration of inoculum was 1x 106 spores per 10 gram of solid materials , initial P H 6.5 and 96 hours of incubation period at 30° C .the enzyme activity was 1300 unit / ml and specific activity was 1550 unit / mg protein.