استخلاص و تنقية منظم النمو حامض الإندول خليك المنتج من العزلة المحلية Fusarium oxysporum (F2)‎

الملخص EN

Indole acetic acid (IAA) produced from F.

oxysporum (F2) was purified by several steps included extraction by cold ethyl acetate ; Column chromatography using silica gel and TLC chromatography .

The pure indole acetic acid (IAA) which produce by F.

oxysporum (IAA) was tested by ultraviolet spectra at (200-300)nm ; and appear that the maximum absorbance at 229nm , the high performance liquid chromatography (HPLC) used to test the purity of the indole acetic acid and the results showed one peak at appearance time 3.822 min.