تقنية إنزيم اليوريز المستخلص من بذور الرقي

الملخص EN

Watermelon seeds showed also a higher production even more than the legumes.

Measured enzyme activity for watermelon milled seeds was found to have 353 unit/gm seeds.

However, stabilization characters were studied which showed that there is a very high stability under extraction conditions and under different storage parameters.

Results also showed that the enzyme stability decreased gradually in the presence of 2-mercaptoethanol, ethylene diamine letra acetate di sodium (EDTA) at (1 mmoiar) for each concentration.

Addition of glycerol (10%) to a buffer's solution used is the process of enzyme activity.

Enzyme from C.

vulgaris was purified through different steps as extraction, dialysis, heat treatment, acetone fractionation, ionic exchange as a (Batch wise) employing ion exchange column using diethyl amino ethyl cellulose (DEAE cellulose),.

Separation results showed that the enzyme found lo have two shapes (A & B) and it can be easil separatcdfrom each other, using NaCI (0.2 and 0.3) Molar subsequently.

The specific activity were measured and found to be (14L66) and (58.57 unit/mg protein) since the activity of enzyme A is superies that of enzymes by two folds.

Purification steps for enzyme A were completed through filtration on the column of Sepbadex G-200 and the specific activity for this purified enzyme reached up to 204.54 unit/mg protein.