التأثيرات المرضية النسجية للراشح الخام لانزيمي ال LasA و البروتيز القاعدي المنتجان من بكتربا Psudomonas aeruginosa على قرنية عيون الأرانب المختبرية

الملخص EN

The species of Pseudomonas aeruginosa was isolated from keratitis.

We found that 12 % were isolated.

The production of elastase were tested.

Four isolated (66.6 %) had the ability to producing elastase.

The production of LasA and alkaline protease were tested, all isolates had the ability to production both the enzymes.

The results of the enjection of alkaline protease crude and LasA enzyme crud in eye corneal rabbits (in vivu) showed that both enzymes had the obvious effect on the cornea.

Histopathological effects on eyes corneal of these experimental rabbits, were seen at concentration of 12 unit \ milliliter of alkaline protease crude and concentration of 7 unit \ milliliter of LasA enzyme crude.

Both enzymes caused epithelium detachment with damage of stromal tissue and formation of oedema.