Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors

المؤلفون المشاركون

Dunlop, James
Phung, Thai
Dalziel, Julie E.
Zhang, Yanli

المصدر

International Journal of Electrochemistry

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-6، 6ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-08-29

دولة النشر

مصر

عدد الصفحات

6

التخصصات الرئيسية

الكيمياء

الملخص EN

To identify materials suitable as membrane supports for ion channel biosensors, six filter materials of varying hydrophobicity, tortuosity, and thickness were examined for their ability to support bilayer lipid membranes as determined by electrical impedance spectroscopy.

Bilayers supported by hydrophobic materials (PTFE, polycarbonate, nylon, and silanised silver) had optimal resistance (14–19 GΩ) and capacitance (0.8–1.6 μF) values whereas those with low hydrophobicity did not form BLMs (PVDF) or were short-lived (unsilanised silver).

The ability of ion channels to function in BLMs was assessed using a method recently reported to improve the efficiency of proteoliposome incorporation into PTFE-supported bilayers.

Voltage-gated sodium channel activation by veratridine and inhibition by saxitoxin showed activity for PTFE, nylon, and silanised silver, but not polycarbonate.

Bilayers on thicker, more tortuous, and hydrophobic materials produced higher current levels.

Bilayers that self-assembled on PTFE filters were the longest lived and produced the most channel activity using this method.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Phung, Thai& Zhang, Yanli& Dunlop, James& Dalziel, Julie E.. 2011. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-454985

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Phung, Thai…[et al.]. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-454985

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Phung, Thai& Zhang, Yanli& Dunlop, James& Dalziel, Julie E.. Porous Materials to Support Bilayer Lipid Membranes for Ion Channel Biosensors. International Journal of Electrochemistry. 2011. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-454985

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-454985