Evaluation of the Electrostatic Contribution to the Retention of Modified Nucleosides and Nucleobases by Zwitterionic Hydrophilic Interaction Chromatography

المؤلفون المشاركون

Rodríguez-Gonzalo, Encarnación
Carabias-Martínez, Rita
García-Gómez, Diego

المصدر

ISRN Analytical Chemistry

العدد

المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-5، 5ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2012-03-15

دولة النشر

مصر

عدد الصفحات

5

التخصصات الرئيسية

الكيمياء

الملخص EN

This work explores the benefits and limitations, on a quantitative basis, of using zwitterionic hydrophilic interaction chromatography (ZIC-HILIC) for the separation of several modified nucleosides and nucleobases of clinical interest.

The target compounds were hydroxylated and methylated derivatives: 8-hydroxy-guanine, 8-hydroxy-guanosine, 8-hydroxy-2′-deoxyguanosine, 1-methyl-guanine, 7-methyl-guanine, and 9-methyl-guanine.

A quantitative evaluation of the electrostatic interaction based on a systematic study of the nature and concentration of the salts in the mobile phase has been carried out.

From the obtained results, it may be concluded that separation is based on a mechanism of partition and interaction through weak electrostatic forces: the contribution of the electrostatic interaction to the retention of the charged analytes reaching values between 25 and 52% at low salt concentration.

However, the electrostatic contribution decreased progressively as the salt concentration rose.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

García-Gómez, Diego& Rodríguez-Gonzalo, Encarnación& Carabias-Martínez, Rita. 2012. Evaluation of the Electrostatic Contribution to the Retention of Modified Nucleosides and Nucleobases by Zwitterionic Hydrophilic Interaction Chromatography. ISRN Analytical Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-462220

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

García-Gómez, Diego…[et al.]. Evaluation of the Electrostatic Contribution to the Retention of Modified Nucleosides and Nucleobases by Zwitterionic Hydrophilic Interaction Chromatography. ISRN Analytical Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-462220

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

García-Gómez, Diego& Rodríguez-Gonzalo, Encarnación& Carabias-Martínez, Rita. Evaluation of the Electrostatic Contribution to the Retention of Modified Nucleosides and Nucleobases by Zwitterionic Hydrophilic Interaction Chromatography. ISRN Analytical Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-462220

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-462220