Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels : Effect of Parameters and Reusability
المؤلفون المشاركون
Divecha, Jyoti
Madamwar, Datta
Shah, Amita
Panchal, Nilam
Raghavendra, Tripti
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-01
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Pentyl valerate was synthesized biocatalytically using Candida rugosa lipase (CRL) immobilized in microemulsion based organogels (MBGs).
The optimum conditions were found to be pH 7.0, temperature of 37°C, ratio of concentration of water to surfactant (Wo) of 60, and the surfactant sodium bis-2-(ethylhexyl)sulfosuccinate (AOT) for MBG preparation.
Although kinetic studies revealed that the enzyme in free form had high affinity towards substrates (Km = 23.2 mM for pentanol and 76.92 mM for valeric acid) whereas, after immobilization, the Km values increased considerably (74.07 mM for pentanol and 83.3 mM for valeric acid) resulting in a slower reaction rate, the maximum conversion was much higher in case of immobilized enzyme (~99%) as compared to free enzyme (~19%).
Simultaneous effects of important parameters were studied using response surface methodology (RSM) conjugated with Box-Behnken design (BBD) with five variables (process parameters), namely, enzyme concentration, initial water content (Wo), solvent used for MBG preparation, substrate ratio and time, and response as the final product formation, that is, pentyl valerate (%).
The MBGs were reused for 10 consecutive cycles for ester synthesis.
Efficacy of AOT/isooctane as dehydrating agent for extracting excess water from MBGs was found to exert a positive effect on the esterification reaction.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Raghavendra, Tripti& Panchal, Nilam& Divecha, Jyoti& Shah, Amita& Madamwar, Datta. 2014. Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels : Effect of Parameters and Reusability. BioMed Research International،Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-465252
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Raghavendra, Tripti…[et al.]. Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels : Effect of Parameters and Reusability. BioMed Research International No. 2014 (2014), pp.1-14.
https://search.emarefa.net/detail/BIM-465252
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Raghavendra, Tripti& Panchal, Nilam& Divecha, Jyoti& Shah, Amita& Madamwar, Datta. Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels : Effect of Parameters and Reusability. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-14.
https://search.emarefa.net/detail/BIM-465252
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-465252
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر