Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium
المؤلفون المشاركون
Stanton, Kristen J.
Wandling, Michael W.
Lu, Xiaoying
Hitchcock-DeGregori, Sarah E.
Kawai, Masataka
المصدر
العدد
المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-17، 17ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2009-10-13
دولة النشر
مصر
عدد الصفحات
17
التخصصات الرئيسية
الملخص EN
Tropomyosin (Tm) consists of 7 quasiequivalent repeats known as “periods,” and its specific function may be associated with these periods.
To test the hypothesis that either period 2 or 3 promotes force generation by inducing a positive allosteric effect on actin, we reconstituted the thin filament with mutant Tm in which either period 2 (Δ2Tm) or period 3 (Δ3Tm) was deleted.
We then studied: isometric tension, stiffness, 6 kinetic constants, and the pCa-tension relationship.
N-terminal acetylation of Tm did not cause any differences.
The isometric tension in Δ2Tm remained unchanged, and was reduced to ~60% in Δ3Tm.
Although the kinetic constants underwent small changes, the occupancy of strongly attached cross-bridges was not much different.
The Hill factor (cooperativity) did not differ significantly between Δ2Tm (1.79 ± 0.19) and the control (1.73 ± 0.21), or Δ3Tm (1.35 ± 0.22) and the control.
In contrast, pCa50 decreased slightly in Δ2Tm (5.11 ± 0.07), and increased significantly in Δ3Tm (5.57 ± 0.09) compared to the control (5.28 ± 0.04).
These results demonstrate that, when ions are present at physiological concentrations in the muscle fiber system, period 3 (but not period 2) is essential for the positive allosteric effect that enhances the interaction between actin and myosin, and increases isometric force of each cross-bridge.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kawai, Masataka& Lu, Xiaoying& Hitchcock-DeGregori, Sarah E.& Stanton, Kristen J.& Wandling, Michael W.. 2009. Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium. Journal of Biophysics،Vol. 2009, no. 2009, pp.1-17.
https://search.emarefa.net/detail/BIM-467540
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kawai, Masataka…[et al.]. Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium. Journal of Biophysics No. 2009 (2009), pp.1-17.
https://search.emarefa.net/detail/BIM-467540
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kawai, Masataka& Lu, Xiaoying& Hitchcock-DeGregori, Sarah E.& Stanton, Kristen J.& Wandling, Michael W.. Tropomyosin Period 3 Is Essential for Enhancement of Isometric Tension in Thin Filament-Reconstituted Bovine Myocardium. Journal of Biophysics. 2009. Vol. 2009, no. 2009, pp.1-17.
https://search.emarefa.net/detail/BIM-467540
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-467540
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر