Anticancer Drug 2-Methoxyestradiol Protects against Renal IschemiaReperfusion Injury by Reducing Inflammatory Cytokines Expression
المؤلفون المشاركون
So, Edmund Cheung
Yeh, Ching-Hua
Chen, Ying-Yin
Wang, Li-Yun
Sun, Ding-Ping
Hsing, Chung-Hsi
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-25
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Ischemia/reperfusion (I/R) injury is a major cause of acute renal failure and allograft dysfunction in kidney transplantation.
ROS/inflammatory cytokines are involved in I/R injury.
2-Methoxyestradiol (2ME2), an endogenous metabolite of estradiol, inhibits inflammatory cytokine expression and is an antiangiogenic and antitumor agent.
We investigated the inhibitory effect of 2ME2 on renal I/R injury and possible molecular actions.
Methods.
BALB/c mice were intraperitoneally injected with 2ME2 (10 or 20 mg/kg) or vehicle 12 h before and immediately after renal I/R experiments.
The kidney weight, renal function, tubular damages, and apoptotic response were examined 24 h after I/R injury.
The expression of mRNA of interleukin-1β, tumor necrosis factor- (TNF) α, caspase-3, hypoxia inducible factor- (HIF) 1α, and proapoptotic Bcl-2/adenovirus E1B 19 kDa interacting protein 3 (BNIP3) in kidney tissue was determined using RT-PCR, while the expression of nuclear factor κB (NF-κB), BCL-2, and BCL-xL, activated caspase-9, and HIF-1α was determined using immunoblotting.
In vitro, we determined the effect of 2ME2 on reactive oxygen species (ROS) production and cell viability in antimycin-A-treated renal mesangial (RMC) and tubular (NRK52E) cells.
Results.
Serum creatinine and blood urea nitrogen were significantly higher in mice with renal I/R injury than in sham control and in I/R+2ME2-treated mice.
Survival in I/R+2ME2-treated mice was higher than in I/R mice.
Histological examination showed that 2ME2 attenuated tubular damage in I/R mice, which was associated with lower expression TNF-α, IL-1β, caspase-9, HIF-1α, and BNIP3 mRNA in kidney tissue.
Western blotting showed that 2ME2 treatment substantially decreased the expression of activated caspase-9, NF-κB, and HIF-1α but increased the antiapoptotic proteins BCL-2 and BCL-xL in kidney of I/R injury.
In vitro, 2MR2 decreased ROS production and increased cell viability in antimycin-A-treated RMC and NRK52E cells.
Conclusions.
2ME2 reduces renal I/R injury in mice because it inhibits the expression of ROS and proinflammatory cytokines and induces antiapoptotic proteins.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chen, Ying-Yin& Yeh, Ching-Hua& So, Edmund Cheung& Sun, Ding-Ping& Wang, Li-Yun& Hsing, Chung-Hsi. 2014. Anticancer Drug 2-Methoxyestradiol Protects against Renal IschemiaReperfusion Injury by Reducing Inflammatory Cytokines Expression. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-471752
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chen, Ying-Yin…[et al.]. Anticancer Drug 2-Methoxyestradiol Protects against Renal IschemiaReperfusion Injury by Reducing Inflammatory Cytokines Expression. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-471752
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chen, Ying-Yin& Yeh, Ching-Hua& So, Edmund Cheung& Sun, Ding-Ping& Wang, Li-Yun& Hsing, Chung-Hsi. Anticancer Drug 2-Methoxyestradiol Protects against Renal IschemiaReperfusion Injury by Reducing Inflammatory Cytokines Expression. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-471752
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-471752
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر