Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells
المؤلفون المشاركون
Chi, Chih-Wen
Chen, Chien-Chih
Chen, Yu-Jen
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-06-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Background.
Armillaridin (AM) is isolated from Armillaria mellea.
We examined the anticancer activity and radiosensitizing effect on human esophageal cancer cells.
Methods.
Human squamous cell carcinoma (CE81T/VGH and TE-2) and adenocarcinoma (BE-3 and SKGT-4) cell lines were cultured.
The MTT assay was used for cell viability.
The cell cycle was analyzed using propidium iodide staining.
Mitochondrial transmembrane potential was measured by DiOC6(3) staining.
The colony formation assay was performed for estimation of the radiation surviving fraction.
Human CE81T/VGH xenografts were established for evaluation of therapeutic activity in vivo.
Results.
AM inhibited the viability of four human esophageal cancer cell lines with an estimated concentration of 50% inhibition (IC50) which was 3.4–6.9 μM.
AM induced a hypoploid cell population and morphological alterations typical of apoptosis in cells.
This apoptosis induction was accompanied by a reduction of mitochondrial transmembrane potential.
AM accumulated cell cycle at G2/M phase and enhanced the radiosensitivity in CE81T/VGH cells.
In vivo, AM inhibited the growth of CE81T/VGH xenografts without significant impact on body weight and white blood cell counts.
Conclusion.
Armillaridin could inhibit growth and enhance radiosensitivity of human esophageal cancer cells.
There might be potential to integrate AM with radiotherapy for esophageal cancer treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chi, Chih-Wen& Chen, Chien-Chih& Chen, Yu-Jen. 2013. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473200
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chi, Chih-Wen…[et al.]. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-473200
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chi, Chih-Wen& Chen, Chien-Chih& Chen, Yu-Jen. Therapeutic and Radiosensitizing Effects of Armillaridin on Human Esophageal Cancer Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-473200
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-473200
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر