A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector
المؤلفون المشاركون
Xiong, Ping
Hu, Futao
Gan, Ning
Li, Tianhua
Zheng, Lei
Cao, Yuting
Wang, Ji
المصدر
Journal of Analytical Methods in Chemistry
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-02-21
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A novel horseradish peroxidase- (HPR-) doped magnetic core-shell Fe3O4@SiO2@Au nanocomposites (Fe-Au MNPs) were employed on immunoassay for the determination of C-reactive protein (CRP) based on a electrochemical quartz crystal microbalance detector (EQCM).
Firstly, the secondary CRP antibody and HRP were both immobilized on the Fe-Au MNPs (Fe-Au MNPs-anti-CRP2/HRP) as a signal tag.
Secondly, the above tag and the primary antibody (anti-CRP1) in the bottom of 96-well microtiter plate were employed to conjugate with a serial of CRP concentrations to produce a sandwich immunocomplex.
Thirdly, the immunocomplex solution was subsequently exposed to 3,3′-diaminobenzidine (DAB) in the presence of H2O2, resulting in an insoluble product.
When the precipitation solution was dripped on EQCM, it can achieve a decrease of frequency of crystal (Δf).
The amount of Δf was proportional to (CRP) from 0.003 to 200 ng mL−1 with a low detection limit of 1 pg mL−1.
Compared with the enzyme-linked immunosorbent assay (ELISA), the immunoassay shows greatly improved sensitivity due to the significant amount of HRP labeled on signal tag.
It also has good specificity and low sample consumption, which is expected to be a benefit for the CRP screening in early diagnosis of cardiovascular disease.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Gan, Ning& Xiong, Ping& Wang, Ji& Li, Tianhua& Hu, Futao& Cao, Yuting…[et al.]. 2013. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-475085
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Gan, Ning…[et al.]. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-475085
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Gan, Ning& Xiong, Ping& Wang, Ji& Li, Tianhua& Hu, Futao& Cao, Yuting…[et al.]. A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-475085
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-475085
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر