Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells

المؤلفون المشاركون

Foerster, Martin
Figulla, Hans Reiner
Wang, Zhenhua
Meusel, Katharina
Franz, Marcus
Jung, Christian
Fritzenwanger, Michael

المصدر

Mediators of Inflammation

العدد

المجلد 2009، العدد 2009 (31 ديسمبر/كانون الأول 2009)، ص ص. 1-7، 7ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-03-10

دولة النشر

مصر

عدد الصفحات

7

التخصصات الرئيسية

الأمراض

الملخص EN

Chronic heart failure (CHF) is associated with elevated concentrations of tumor necrosis factor (TNF) α and cardiotrophin-1 (CT-1) and altered peripheral blood mononuclear cell (PBMC) function.

Therefore, we tested whether CT-1 induces TNFα in PBMC of healthy volunteers.

CT-1 induced in PBMC TNFα protein in the supernatant and TNFα mRNA in a concentration- and time-dependent manner determined by ELISA and real-time PCR, respectively.

Maximal TNFα protein was achieved with 100 ng/mL CT-1 after 3–6 hours and maximal TNFα mRNA induction after 1 hour.

ELISA data were confirmed using immunofluorescent flow cytometry.

Inhibitor studies with actinomycin D and brefeldin A showed that both protein synthesis and intracellular transport are essential for CT-1 induced TNFα expression.

CT-1 caused a dose dependent nuclear factor (NF) κB translocation.

Parthenolide inhibited both NFκB translocation and TNFα protein expression indicating that NFκB seems to be necessary.

We revealed a new mechanism for elevated serum TNFα concentrations and PBMC activation in CHF besides the hypothesis of PBMC activation by bacterial translocation from the gut.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Fritzenwanger, Michael& Meusel, Katharina& Jung, Christian& Franz, Marcus& Wang, Zhenhua& Foerster, Martin…[et al.]. 2010. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-475710

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Fritzenwanger, Michael…[et al.]. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-475710

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Fritzenwanger, Michael& Meusel, Katharina& Jung, Christian& Franz, Marcus& Wang, Zhenhua& Foerster, Martin…[et al.]. Cardiotrophin-1 Induces Tumor Necrosis Factor α Synthesis in Human Peripheral Blood Mononuclear Cells. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-475710

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-475710