PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells

المؤلفون المشاركون

Wang, Xian
Li, Fuwang
Hong, Tian-pei
Lu, Changlin
An, Huijie
Wang, Haining
Wang, Guang
Liu, Jinbo
Hao, Yanting
Chen, Alex F.
He, Liyun

المصدر

PPAR Research

العدد

المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2011-11-16

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)
الأحياء

الملخص EN

Tetrahydrobiopterin (BH4) is an essential cofactor for endothelial nitric oxide (NO) synthase.

Guanosine 5′-triphosphate cyclohydrolase-I (GTPCH-I) is a key limiting enzyme for BH4 synthesis.

In the present in vitro study, we investigated whether peroxisome proliferator-activated receptor α (PPAR-α) agonist fenofibrate could recouple eNOS by reversing low-expression of intracellular BH4 in endothelial cells and discussed the potential mechanisms.

After human umbilical vein endothelial cells (HUVECs) were treated with lipopolysaccharide (LPS) for 24 hours, the levels of cellular eNOS, BH4 and cell supernatant NO were significantly reduced compared to control group.

And the fluorescence intensity of intracellular ROS was significantly increased.

But pretreated with fenofibrate (10 umol/L) for 2 hours before cells were induced by LPS, the levels of eNOS, NO, and BH4 were significantly raised compared to LPS treatment alone.

ROS production was markedly reduced in fenofibrate group than LPS group.

In addition, our results showed that the level of intracellular GTPCH-I detected by western blot was increased in a concentration-dependent manner after being treated with fenofibrate.

These results suggested that fenofibrate might help protect endothelial function and against atherosclerosis by increasing level of BH4 and decreasing production of ROS through upregulating the level of intracellular GTPCH-I.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Liu, Jinbo& Lu, Changlin& Li, Fuwang& Wang, Haining& He, Liyun& Hao, Yanting…[et al.]. 2011. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478434

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Liu, Jinbo…[et al.]. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-478434

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Liu, Jinbo& Lu, Changlin& Li, Fuwang& Wang, Haining& He, Liyun& Hao, Yanting…[et al.]. PPAR-α Agonist Fenofibrate Upregulates Tetrahydrobiopterin Level through Increasing the Expression of Guanosine 5′-Triphosphate Cyclohydrolase-I in Human Umbilical Vein Endothelial Cells. PPAR Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-478434

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-478434