LC-NMR Technique in the Analysis of Phytosterols in Natural Extracts

الملخص EN

The ability of LC-NMR to detect simultaneously free and conjugated phytosterols in natural extracts was tested.

The advantages and disadvantages of a gradient HPLC-NMR method were compared to the fast composition screening using SEC-NMR method.

Fractions of free and conjugated phytosterols were isolated and analyzed by isocratic HPLC-NMR methods.

The results of qualitative and quantitative analyses were in a good agreement with the literature data.