Modulation of Apoptosis Pathways by Oxidative Stress and Autophagy in β Cells
المؤلفون المشاركون
Pugazhenthi, Subbiah
Wang, Maorong
Crager, Mia
المصدر
Experimental Diabetes Research
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-03-12
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
Human islets isolated for transplantation are exposed to multiple stresses including oxidative stress and hypoxia resulting in significant loss of functional β cell mass.
In this study we examined the modulation of apoptosis pathway genes in islets exposed to hydrogen peroxide, peroxynitrite, hypoxia, and cytokines.
We observed parallel induction of pro- and antiapoptotic pathways and identified several novel genes including BFAR, CARD8, BNIP3, and CIDE-A.
As BNIP3 is an inducer of autophagy, we examined this pathway in MIN6 cells, a mouse beta cell line and in human islets.
Culture of MIN6 cells under low serum conditions increased the levels of several proteins in autophagy pathway, including ATG4, Beclin 1, LAMP-2, and UVRAG.
Amino acid deprivation led to induction of autophagy in human islets.
Preconditioning of islets with inducers of autophagy protected them from hypoxia-induced apoptosis.
However, induction of autophagy during hypoxia exacerbated apoptotic cell death.
ER stress led to induction of autophagy and apoptosis in β cells.
Overexpression of MnSOD, an enzyme that scavenges free radicals, resulted in protection of MIN6 cells from cytokine-induced apoptosis.
Ceramide, a mediator of cytokine-induced injury, reduced the active phosphorylated form of Akt and downregulated the promoter activity of the antiapoptotic gene bcl-2.
Furthermore, cytokine-stimulated JNK pathway downregulated the bcl-2 promoter activity which was reversed by preincubation with SP600125, a JNK inhibitor.
Our findings suggest that β cell apoptosis by multiple stresses in islets isolated for transplantation is the result of orchestrated gene expression in apoptosis pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Maorong& Crager, Mia& Pugazhenthi, Subbiah. 2012. Modulation of Apoptosis Pathways by Oxidative Stress and Autophagy in β Cells. Experimental Diabetes Research،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-487978
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Maorong…[et al.]. Modulation of Apoptosis Pathways by Oxidative Stress and Autophagy in β Cells. Experimental Diabetes Research No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-487978
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Maorong& Crager, Mia& Pugazhenthi, Subbiah. Modulation of Apoptosis Pathways by Oxidative Stress and Autophagy in β Cells. Experimental Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-487978
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-487978
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر