Dephosphorylation of Centrins by Protein Phosphatase 2C α and β

الملخص EN

In the present study, we identified protein phosphatases dephosphorylating centrins previously phosphorylated by protein kinase CK2.

The following phosphatases known to be present in the retina were tested: PP1, PP2A, PP2B, PP2C, PP5, and alkaline phosphatase.

PP2C α and β were capable of dephosphorylating P-Thr138-centrin1 most efficiently.

PP2Cδ was inactive and the other retinal phosphatases also had much less or no effect.

Similar results were observed for centrins 2 and 4.

Centrin3 was not a substrate for CK2.

The results suggest PP2C α and β to play a significant role in regulating the phosphorylation status of centrins in vivo.