Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice
المؤلفون المشاركون
Lu, Jie
Lin, Chunlei
Wang, Fan
Shen, Miao
Xu, Xuanfu
Cheng, Ping
He, Lei
Zhou, Ying-Qun
Guo, Chuan-Yong
Wang, Chengfen
Xu, Ling
Weiqi, Dai
المصدر
العدد
المجلد 2013، العدد 2013 (31 ديسمبر/كانون الأول 2013)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2013-10-01
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Objective.
Necrostatin-1 (Nec-1) inhibits receptor-interacting protein 1 (RIP1) kinase and programmed necrosis.
This study was designed to examine the protective effects and mechanisms of Nec-1 in concanavalin A- (ConA-) induced hepatitis in mice.
Methods.
C57BL/6 mice were exposed to ConA via tail vein injection and injected intraperitoneally with Nec-1 or vehicle.
Levels of serum liver enzymes and histopathology were determined.
Levels of inflammatory cytokines with ConA-induced hepatitis were determined with real-time polymerase chain reaction (real-time PCR).
The expression of TNF-α, RIP1, and LC3 was detected with immunohistochemical staining.
The expression of TNF-α, IFN-γ, IL2, IL6, caspase 3, RIP1, beclin-1, and LC3 protein was assessed by immunofluorescence and western blotting.
Autophagosomes were observed with transmission electron microscopy (TEM).
Results.
Amelioration in liver functions and histopathological changes and the suppression of inflammatory cytokine production were observed in Nec-1-injected mice.
Western blotting analysis showed that the expression of TNF-α, IFN-γ, IL2, IL6, and RIP1 was significantly reduced in the Nec-1-injected mice, which was confirmed by immunofluorescence and immunohistochemistry.
Autophagosome formation was significantly reduced by Nec-1 treatment, as the expression of beclin-1 and LC3, determined with immunofluorescence and western blotting.
Conclusion.
These results demonstrate that Nec-1 prevents ConA-induced liver injury via RIP1-related and autophagy-related pathways.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhou, Ying-Qun& Weiqi, Dai& Lin, Chunlei& Wang, Fan& He, Lei& Shen, Miao…[et al.]. 2013. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-492072
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhou, Ying-Qun…[et al.]. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation No. 2013 (2013), pp.1-15.
https://search.emarefa.net/detail/BIM-492072
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhou, Ying-Qun& Weiqi, Dai& Lin, Chunlei& Wang, Fan& He, Lei& Shen, Miao…[et al.]. Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-15.
https://search.emarefa.net/detail/BIM-492072
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-492072
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر