Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells
المؤلفون المشاركون
Farfan, Mauricio J.
Izquierdo, Mariana
Nataro, James P.
Ruiz-Perez, Fernando
Alvestegui, Alejandra
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-08-07
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Adherence to the intestinal epithelia is a key feature in enteroaggregative Escherichia coli (EAEC) infection.
The aggregative adherence fimbriae (AAFs) are involved in EAEC interaction with receptors at the surface of intestinal cells.
We and others have demonstrated that fibronectin is a receptor for AAF/II fimbriae.
Considering that the major cellular receptor of fibronectin is integrin α5β1, in this study we evaluated the participation of this receptor in the fibronectin-mediated adherence of EAEC strain 042 to intestinal cells.
We found that EAEC strain 042 has the ability to bind directly and indirectly to integrin α5β1; direct binding was not mediated by AAF/II fimbriae and indirect binding was mediated by AAF/II and fibronectin.
Coimmunoprecipitation assays confirmed the formation of the complex AafA/fibronectin/integrin α5β1.
To evaluate EAEC adherence to intestinal cells, T84 cells were incubated with fibronectin and an antibody that blocks the interaction region of integrin α5β1 to fibronectin, the RGD site.
Under these conditions, we found the number of adherent bacteria to epithelial cells significantly reduced.
Additionally, fibronectin-mediated adherence of EAEC strain 042 was abolished in HEp-2 cells transfected with integrin α5 shRNA.
Altogether, our data support the involvement of integrin α5β1 in the fibronectin-mediated EAEC binding to intestinal cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Izquierdo, Mariana& Alvestegui, Alejandra& Nataro, James P.& Ruiz-Perez, Fernando& Farfan, Mauricio J.. 2014. Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-497482
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Izquierdo, Mariana…[et al.]. Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-497482
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Izquierdo, Mariana& Alvestegui, Alejandra& Nataro, James P.& Ruiz-Perez, Fernando& Farfan, Mauricio J.. Participation of Integrin α5β1 in the Fibronectin-Mediated Adherence of Enteroaggregative Escherichia coli to Intestinal Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-497482
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-497482
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر