GADD45β Determines Chemoresistance and Invasive Growth of Side Population Cells of Human Embryonic Carcinoma

المؤلفون المشاركون

Takeuchi, Takumi
Kitamura, Tadaichi
Aburatani, Hiroyuki
Fujita, Toshiro
Inowa, Toshihiko
Matsuzaki, Yumi
Isagawa, Takayuki
Hishikawa, Keiichi

المصدر

Stem Cells International

العدد

المجلد 2010، العدد 2010 (31 ديسمبر/كانون الأول 2010)، ص ص. 1-10، 10ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2010-03-14

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

العلوم الطبيعية والحياتية (متداخلة التخصصات)

الملخص EN

Side population (SP) cells are an enriched population of stem, and the existence of SP cells has been reported in human cancer cell lines.

In this study, we performed an SP analysis using 11 human cancer cell lines and confirmed the presence of SP cells in an embryonic carcinoma cell line, NEC8.

NEC8 SP cells showed characteristics of cancer stem cells, such as high growth rate, chemoresistance and high invasiveness.

To further characterize the NEC8 SP cells, we used DNA microarrays.

Among 38,500 genes, we identified 12 genes that were over-expressed in SP cells and 1 gene that was over-expressed in non-SP cells.

Among these 13 genes, we focused on GADD45b.

GADD45b was over-expressed in non-SP cells, but the inhibition of GADD45b had no effect on non-SP cells.

Paradoxically, the inhibition of GADD45b significantly reduced the viability of NEC8 SP cells.

The inhibition of ABCG2, which determines the SP phenotype, had no effect on the invasiveness of NEC8 SP cells, but the inhibition of GADD45b significantly reduced invasiveness.

These results suggest that GADD45b, but not ABCG2, might determine the cancer stem cell-like phenotype, such as chemoresistance and the high invasiveness of NEC8 SP cells, and might be a good therapeutic target.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Inowa, Toshihiko& Hishikawa, Keiichi& Matsuzaki, Yumi& Isagawa, Takayuki& Takeuchi, Takumi& Aburatani, Hiroyuki…[et al.]. 2010. GADD45β Determines Chemoresistance and Invasive Growth of Side Population Cells of Human Embryonic Carcinoma. Stem Cells International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-497680

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Inowa, Toshihiko…[et al.]. GADD45β Determines Chemoresistance and Invasive Growth of Side Population Cells of Human Embryonic Carcinoma. Stem Cells International No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-497680

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Inowa, Toshihiko& Hishikawa, Keiichi& Matsuzaki, Yumi& Isagawa, Takayuki& Takeuchi, Takumi& Aburatani, Hiroyuki…[et al.]. GADD45β Determines Chemoresistance and Invasive Growth of Side Population Cells of Human Embryonic Carcinoma. Stem Cells International. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-497680

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-497680