Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits
المؤلفون المشاركون
Sasaki, Tomio
Matsuo, Satoshi
Nakamizo, Akira
Kurogi, Ryota
Mizoguchi, Masahiro
Kikkawa, Yuichiro
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-07-16
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Background.
Although relaxin causes vasodilatation in systemic arteries, little is known about its role in cerebral arteries.
We investigated the expression and role of relaxin in basilar arteries after subarachnoid hemorrhage (SAH) in rabbits.
Methods.
Microarray analysis with rabbit basilar artery RNA was performed.
Messenger RNA expression of relaxin-1 and relaxin/insulin-like family peptide receptor 1 (RXFP1) was investigated with quantitative RT-PCR.
RXFP1 expression in the basilar artery was investigated with immunohistochemistry.
Relaxin concentrations in cerebrospinal fluid (CSF) and serum were investigated with an enzyme-linked immunosorbent assay.
Using human brain vascular smooth muscle cells (HBVSMC) preincubated with relaxin, myosin light chain phosphorylation (MLC) was investigated with immunoblotting after endothelin-1 stimulation.
Results.
After SAH, RXFP1 mRNA and protein were significantly downregulated on day 3, whereas relaxin-1 mRNA was significantly upregulated on day 7.
The relaxin concentration in CSF was significantly elevated on days 5 and 7.
Pretreatment with relaxin reduced sustained MLC phosphorylation induced by endothelin-1 in HBVSMC.
Conclusion.
Upregulation of relaxin and downregulation of RXFP1 after SAH may participate in development of cerebral vasospasm.
Downregulation of RXFP1 may induce a functional decrease in relaxin activity during vasospasm.
Understanding the role of relaxin may provide further insight into the mechanisms of cerebral vasospasm.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kikkawa, Yuichiro& Matsuo, Satoshi& Kurogi, Ryota& Nakamizo, Akira& Mizoguchi, Masahiro& Sasaki, Tomio. 2014. Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502017
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kikkawa, Yuichiro…[et al.]. Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-502017
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kikkawa, Yuichiro& Matsuo, Satoshi& Kurogi, Ryota& Nakamizo, Akira& Mizoguchi, Masahiro& Sasaki, Tomio. Upregulation of Relaxin after Experimental Subarachnoid Hemorrhage in Rabbits. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-502017
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-502017
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر