A Novel Utility for an Old Method : The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays
المؤلفون المشاركون
Silva, Mariana Dias da
Argentão, Daiana Guedes Pinto
Santos, Raquel Acácia Pereira Gonçalves dos
Lima, Regiane Patussi Santos
Silva, Grayce Katlen Moreno da
Olivier, Celso Eduardo
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-02-18
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
The leukocyte adherence inhibition (LAI) test was designed to evaluate the presence of cellular immunoreactivity against specific antigens and is highly sensitive to analytical interference.
To compare the performance of the LAI test among plasmas obtained with different anticoagulants, 36 blood samples of allergic subjects were divided into three tubes with heparin, sodium citrate, and EDTA.
The adherence was assessed with the hemocytometer technique.
The mean adherence of each group was compared as well as the number of tests with inappropriate adherence (<25%).
Of the 36 tests, there were 4, 9, and 21 with inappropriate adherence in the groups of heparin, citrate, and EDTA, respectively.
Of these last, 5 samples also did not demonstrate proper adherence with sodium citrate and 1 sample did not show proper adherence with any anticoagulant.
The anticoagulant in the collection tube can elicit a specific immune response, becoming an interferent for the LAI test.
In this case, there is the possibility of redoing the test using another anticoagulant.
Performed with different anticoagulants, the LAI test may be used as a preanalytical tool for choosing the less immunoreactive anticoagulant for more complex immunoassays.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Olivier, Celso Eduardo& Santos, Raquel Acácia Pereira Gonçalves dos& Lima, Regiane Patussi Santos& Argentão, Daiana Guedes Pinto& Silva, Grayce Katlen Moreno da& Silva, Mariana Dias da. 2014. A Novel Utility for an Old Method : The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays. Journal of Cell Adhesion،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-504082
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Olivier, Celso Eduardo…[et al.]. A Novel Utility for an Old Method : The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays. Journal of Cell Adhesion No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-504082
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Olivier, Celso Eduardo& Santos, Raquel Acácia Pereira Gonçalves dos& Lima, Regiane Patussi Santos& Argentão, Daiana Guedes Pinto& Silva, Grayce Katlen Moreno da& Silva, Mariana Dias da. A Novel Utility for an Old Method : The Leukocyte Adherence Inhibition Test Is an Easy Way to Detect the Immunoreactive Interference of the Collection Tube Anticoagulant on Cellular Immunoassays. Journal of Cell Adhesion. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-504082
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-504082
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر