حصر المجموعات الرئيسية من ذبابة التبغ البيضاء Bemisia tabaci Genn. المنتشرة على عوائل مختلفة في الساحل السوري باستخدام مؤشرات الـ DNA الدنا العشوائية

الملخص EN

Genetic diversity of Bemisia tabaci Genn.

was detected in 44 populations collected from different hosts in the Syrian Coast by using RAPD-PCR.

The number of polymorphic fragments was 146 with polymorphism rate of 86.39%, and the highest number of amplified fragments was 29 bands with 93.1% polymorphism when the primer OPA-13 was used, whereas the highest polymorphism was 100% when primers OPF-12 and OPH-09 were used.

The rate of polymorphic loci (PPB) for all studied populations was 48%, the number of alleles per locus (N0) was 2.085, the effective number of alleles (NE) was 1.33, and the genetic diversity (HE) was 0.151.

The phylogenetic tree showed that the studied populations were divided into two groups according to altitude at similarity rate of 48%.

The highest genetic similarity was 85% between Bt-8 and Bt-14, whereas the least genetic similarity was 39% between Bt-38 and Bt-9 populations.

In addition, the collected populations were distributed to three classes (C1, C2, C3) depending on its environment, and Shannon's index for these classes was 2.771, 2.769 and 2.483, respectively.

There was not any correlation between plant hosts and genetic diversity for these population (R2=0.016).