إنتاج مصل مضاد متعدد الكلون لفيروس التبرقش الريشي على البطاطا الحلوة (SPFMV)‎ في سورية

الملخص EN

This study aimed to purify a local isolate of Sweet potato feathery mottle virus (SPFMV, Potyvirus; Potyviridae), and produce a polyclonal antiserum against it.

SPFMV was reported to be the main virus affecting sweet potato fields in Syria.

Results showed that, SPFMV can be purified directly from sweet potato and from I.

setosa infected leaves.

The amount of purified virus varied based on infected host species, harvest time, and storage duration under -80°C.

Moreover, I.

setosa leaves yielded 1.1-6.4 mg/kg compared with 0.06-3.0 mg/kg from sweet potato infected leaves.

SPFMV antiserum was produced against a Syrian isolate of SPFMV and used to reliably identify the virus in SPFMV-infected samples collected from Syria.

The results of using this antibody were consistent with those obtained from using SPFMV antibody obtained from the International Potato Center (CIP).

In addition, the locally produced antibody was specific to SPFMV, sensitive, and detected SPFMV infection in plant extract diluted 1:500.The low cost of producing SPFMV antiserum locally will permit conducting large scale surveys for the virus and facilitate the production of virus free sweet potato propagation material in Syria.