تقييم كفاءة عديد السكرايد المحفظي المستخلص من بكتريا Proteus vulgaris في تحفيز الجهاز المناعي و كمضاد مايكروبي

الملخص EN

The current study including two parts : The first was determination antimicrobial effect of Proteus vulgaris on two type of pathogenic bacteria such as S.aureus and P.

aeruginosa, the second was evaluate the effect of capsular polysaccharide antigen which extract from Proteus vulgaris to stimulate immune response in mice.

CPS antigen from P.

vulgaris was prepared, then series concetration of wild type of bathogenic bacteria and from CPS antigen were prepared depending on LD50 value which was ; 1 × 105 cfu / ml of wilde type and 1 × 108 cfu / ml of CPS antigens, these concentration injected in 0.1 ml intraperetonial in mice.

Immune response in mice evaluated by using the following assay : (phagocytosis, Arthus reaction, delayed hypersensitivity, T-rossate formation and plaque forming cells) by use two concentration LD50 and 0.1 LD50 which was (1 × 108, 1 × 107) cfu / ml in 0.1 ml from CPS antigens « to evaluate the best concentration in stimulate immune response» and bacterial suspention in 3 mice to each test in addition to control group injected by normal saline.

The results of this study confirmed that, the P.

vulgaris was effective in inhibiting the growth of investigated bacteria, with inhibition zone 28 mm for S.aureus and 23 mm for P.aeruginosa, also the result show that the CPS antigen in 1 × 107 cfu / ml concentration after 14 days from injection is the best efficient and gave highest mean in phagocytosis, delayed hypersansativity, T-rossate formation and, specific immunity in term of plaque forming cells and arthus reaction was significantly increase in 1 × 107 cfu / ml.

It is concluded that efficient immune response can be obtained by CPS from P.

vulgaris.