استعمال المانيتول المنتج من عزلة محلية لبكتيريا Leuconostoc mesenteroides في تحضير الوسط الزرعي mannitol salt agar

الملخص EN

Leuconostoc mesenteroides pure isolate was obtained from Purslane plant (oleracea Portulasa), It which grown on mannitol media production contained on date extract.

The yield of mannitol production yield was 12.8 g / 100 ml that identified by paper chromot agraphy which revealed that Rf value of standard mannitol and produced mannitol were equal .The results of infra-red spectra (IR) analysis of produced mannitol were compatible to that of the Aldrich library of Infra-red spectra of mannitol.

The produced mannitol was used to prepare mannitol salt agar for cultivation of Staphylococcus aureus using pour plate and spread methods which changed the medium color, also colonies numbers and characters approached those which grew on standard mannitol salt agar medium.