مقارنة استخدام الأوساط الزرعية الروتينية و المحوره للكشف عن البكتيريا فاقدة الجدار و المعزولة من خمج السبيل البولي

الملخص EN

Has been studied cryptic bacterial infection in the urinary tract to humans.

50 urine samples were processed patients Auditors advisory Hospital Hilla public education for the detection cell wall Defectivec Bacteria (CWDB) for the period between November 2012 until February 2013.

Samples were distributed evenly between the sexes and aged between 20-50 years.

The samples were collected in standards way and renounced device speed centrifugation 3000 c / m for 5 minutes to precipitate the contents and examined under a microscope.

Found that the cellular response non-specialized to precipitate the urine was Mixed (34 %) and monocytic (30 %).

Samples were planted on routines media (blood agar & macchoncky agar) and modified (variant broth & agar) and incubated for 24-48 hours at a temperature 37c.

Out put diagnosed by bacterial system diagnostics, phenotypic, microscopically and biochemistry and using API 20 E and the use VITEK 2 to confirm some samples results.

Growth on Variant agar yielded 70 % growth of CWDB and intact, while growth on usuall media yielded 32 % of intact bacteria.

The percent of CWDB among growths on variant agar was 38 %.

and the results showed 30 % negative situation.

Citrobacter freundii recorded 12 %, the highest rate among cell-wall defective isolates.

Drug sensitivity were screened isolates from U.T.I both two type (CWB & CWDB) the highest rate of sensitivity to the antibiotic Impinem represented CWDB, as well as the highest rate of resistance to Ampicillin represented by the same group of isolates.