تأثير فعالية البكتريا Bacillus subtilis و Pseudomonas florescens في تثبيط فعل الفطر Macrophomina phaseolina المسبب لمرض التعفن الفحمي على الفاصوليا

الملخص EN

This study aimed to isolation of Macrophomina phaseolina (tassi) goid.

fungus which cause Charcol rot disease on beans, from jbala district / Babylon governorate, diagnose, pathogenicity ability test, and evaluate efficacy of two factors of biological control agents Bacillus subtilis, Pseudomonas florescens, its effect on pathogenic fungus under laboratory and lath house conditions.

The results of isolation and Microscopic diagnose showed the presence of M.

phaseolina fungus in almost samples of infected bean.

the results of pathogenicity showed the ability of M.

phaseolina to reduce the rate of Radish seeds germination which was 6 % compared with control with out pathogen 94 %, antagonist ability test of bacteria B.

subtilis and P.

florescens with different dilution rates (1-1- 10-10) against pathogenic fungus isolate on PSA medium the rate 10-1 for bacteria B.

subtilis has ability to inhibit pathogenic fungus as 47.21 %, while in bacteria P.

florescens in rate 10-6 has ability to inhibit pathogenic fungus in 100 % ratio compared with control.

Which inhibition ratio was 0.0 %.

The results showed that the P.

florescens with different concentrations increased the bean seed germination under lath house condition, which was 90 % and decrease the percentage ratio of bean charcoal rot disease incidence to 44.66 % and severity to 21.66 %.

This increased wet and dry weight of bean seedlings.