دراسة التنوع الوراثي للقصب البري Phragmites australis في العراق باعتماد تقنية RAPD

الملخص EN

Genetic diversity was studied in 31 Iraqi common reed samples, which were collected from Iraqi marshes in Basrah, Messan and Thi-Qar provinces and also from different areas in Baghdad province.

Random amplified polymorphic DNA (RAPD) technique was used for evaluation of genetic diversity between collected samples.

Seven primers were used for polymorphism detecting between common reed samples.

The results revealed 102 bands for the all samples when RAPD-PCR was used.

The percentage rate for the monomorphic bands is 6.86 %, while the percentage rate for the polymorphic bands is 93.13 %, and the numbers of these bands are ranging between 10 to 17 for each used primer.

The UBC1 primer gave the highest number of polymorphic bands with 17 bands, while the M13 and Op-B01 primers gave the lowest number of polymorphic bands with 10 bands.

The mean value of polymorphic bands is 13.5 for each used primer, while the percentage mean ratio for polymorphism is 92.67 %, ranging between 71.42 % for M13 primer and 100 % for UBC1 and Op-B11 primers.

The results of dendrogram analysis for the RAPD data by using unweighted pair-group method with arithmetic average (UPGMA) were showed that almost common reed samples were distributed in six main groups with 60 % in similarity.

These six groups showed coincidence with their geographic area.

The samples which collected from one location or from same province were clustered in one group, while the Meshab and Sallal (Basrah) isolated samples were segregated from the other samples.

The genetic variations between isolated common reeds have advantage in weeds control by using chemical or other methods.