تنميط فيروس مرض التهاب الجراب المعدي باستعمال اختبار الأليزا اللاقطة للأضداد وحيدة النسيلة

الملخص EN

Bursae of Fabricius were collected from 35 broiler flocks naturally infected with infectious bursal disease (IBD) in three areas of Syria (north, middle, south) during the period from 2006 to 2008.

The aim was to detect the presence of the virus, identify virus strain and to study morbidity and mortality rates.

The study revealed that the morbidity rate ranged between 6 to 70% and the mortality rate ranged between 1 to 40%.

The infection rate was higher in chickens aged 18-37 days and lower in chickens aged 40 days and more.

The IBD virus was isolated and propagated by inoculation on the chorioallantoic membrane (CAM) of specific pathogen free (SPF) chicken embryos.

The viruses in bursae of Fabricius supernatant were screened and typed by Capture- ELISA using four monoclonal antibodies and compared with classic and variant strains (E/Del,GLS,RS593).

Results showed that twenty nine of isolates were of the classical form.