In vitro study the affinity of sickle cell hemoglobin to change to methaemoglobin

الملخص EN

This study involve (80) female medical student, aged between(19-24) years, subdivided into three groups, control group that consist of (30) female with normal hemoglobin erythrocyte genotype(Hb AA) and two case groups, each one consist of (25) female, one with(Hb AS) and the other with(HbSS) erythrocyte genotype.

In this study we tried to identify the affinity of sickle erythrocyte hemoglobin to change to methaemoglobin (MetHb) in three human erythrocyte genotypes, namely,( HbAS) and (HbSS) compared with normal genotype (HbAA) .

The cyanomethaemoglobin reaction was used for the determination of erythrocyte haemolysate methaemoglobin concentration.

The mean ± SD methaemoglobin concentration, expressed as percentage (MetHb%) of total Hb erythrocyte genotypes (HbAS,HbSS and HbAA).

Thus, the oxidative damage of Hb diminishes among the studied genotypes in the manner: SS>AS>AA.

We demonstrate a statistically significant differences (P<0.05) in MetHb% between (HbSS) and (HbAA) erythrocyte genotypes, whereas, there is no significant differences (P>0.05) in MetHb% between HbAS and HbAA erythrocyte genotypes.

This results may provide further insight into the mechanism of how (MethHb)formation benefit sickle cell patients and may offer an attractive new therapeutic model for the treatment of sickle cell disease.