Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the Teaching Hospital of Kashan, Iran
المؤلفون المشاركون
Amiri, Atena
Moniri, Rezvan
Firoozeh, Farzaneh
Zibaei, Mohammad
المصدر
Iranian Red Crescent Medical Journal
العدد
المجلد 18، العدد 2 (28 فبراير/شباط 2016)، ص ص. 1-7، 7ص.
الناشر
تاريخ النشر
2016-02-28
دولة النشر
الإمارات العربية المتحدة
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Background: Extended-spectrum β-lactamases (ESBLs) is one of the most important mechanisms of resistance to β-lactams especially among Enterobacteriaceae family including Klebsiella spp.
Different types of extended-spectrum β-lactamases including CTX-M-type and PER enzymes are identified among gram negative bacteria.
Objectives: The current study aimed to determine the prevalence of CTX-M-type and PER extended-spectrum β-lactamases among Klebsiella spp.
isolated from clinical specimens in the teaching hospital of Kashan, Iran.
Patients and Methods: One hundred Klebsiella spp.
were isolated from clinical specimens of hospitalized patients at Shahid-Beheshti hospital from December 2012 to November 2013.
Disk diffusion method was used to determine the susceptibility of these isolates to 14 different antimicrobial agents; disks were purchased from MAST company (United Kingdom).
The phenotypic double disk synergy confirmatory test was used to screen the isolates to produce extended-spectrum β-lactamase.
DNAs of isolates were extracted using boiling method and PCR assay was used to characterize the blaCTX-M type and blaPER genes.
The purified PCR products were sent to Macrogen research company (Korea) for sequencing.
Results: Of the total 100 Klebsiella isolates, %93 was susceptible to imipenem.
Resistance to ampicillin, ceftazidime, ceftriaxone, aztreonam and cefotaxime was (92%), (67%), (65%), (64%) and (59%), respectively.
The phenotypic confirmatory test (PCT) confirmed that 35% (n = 35) of the isolates were ESBL-producing Klebsiella strains.
The prevalence of blaCTX-M type and blaRER genes among Klebsiella isolates were 28% (n = 28) and 9% (n = 9), respectively.
Conclusions: The prevalence of ESBL-producing Klebsiella strains in Shahid-Beheshti hospital in Kashan has increased.
The study concluded that there was a high prevalence of the blaCTX-M type gene among ESBL positive isolates.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Amiri, Atena& Firoozeh, Farzaneh& Moniri, Rezvan& Zibaei, Mohammad. 2016. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the Teaching Hospital of Kashan, Iran. Iranian Red Crescent Medical Journal،Vol. 18, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-654837
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Amiri, Atena…[et al.]. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the Teaching Hospital of Kashan, Iran. Iranian Red Crescent Medical Journal Vol. 18, no. 2 (Feb. 2016), pp.1-7.
https://search.emarefa.net/detail/BIM-654837
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Amiri, Atena& Firoozeh, Farzaneh& Moniri, Rezvan& Zibaei, Mohammad. Prevalence of CTX-M-Type and PER extended-spectrum β-lactamases among klebsiella spp. isolated from clinical specimens in the Teaching Hospital of Kashan, Iran. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 2, pp.1-7.
https://search.emarefa.net/detail/BIM-654837
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 6-7
رقم السجل
BIM-654837
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر