انتشار طفرة FLT3 (D835)‎ لدى مرضى سوريين مصابين بابيضاض دم نقوي حاد من مختلف النميطات

الملخص EN

Acute myeloid leukemia (AML) is characterized by an increase in the number of myeloid cells in the marrow and an arrest in their maturation.

Many mutations play a role in this disease, Fms-like tyrosine kinase 3 (FLT3) gene mutations is one of these mutations.

FLT3 gene, a member of the class III receptor tyrosine kinase family, plays an important role in hematopoietic stem cell survival, development and differentiation.

Mutations in Fms-like tyrosine kinase 3 genes lead to uncontrolled proliferation of leukemic cells with a poor prognosis.

AML patients with FLT3 internal tandem duplication (ITD) mutations have poor cure rate, the prognostic significance of tyrosine kinase domain (TKD)\ D835 is still unclear.

Mutations of FLT3 were first described in 1997 and account for the most frequent molecular mutations in acute myeloid leukemia (AML).There is no sufficient data currently exist about FLT3 mutations in Syrian AML patients.

So, our study aimed to determine the frequency of FLT3 (D835) mutation and study the correlation between this mutation and AML subtypes, according to (FAB) French American British classification, in 70 relapsed AML patients (36 males and 34 females), by using PCR followed by digestion with EcoRV enzyme.

Frequency of FLT3 (D835) mutation in our study was 4.3%.

This study revealed no significant correlation between FLT3 mutation and age and sex.

And no correlation between this mutation and any AML subtypes.