التوصيف الجزيئي لنباتات متوسطة الطول في الشعير باستخدام تقنية RSS

الملخص EN

This investigation was carried out at the Laboratory of Biotechnology, Faculty of Agriculture, Damascus University, during the season 2010/ 2011.

Barley variety (fourat 1) treated seeds exposed to Gamma ray with 10 kilorad dose and untreated seeds were sown to identify the presence of genetic loci for lodging resistance and to determine the degree of genetic relationship using the SSR technique (Simple Sequence Repeats).

All primers proved their effectiveness in showing polymorphism between studied individuals, and gave a total of 12 alleles with a polymorphic percentage of 94.44%.

The number of bands for each primer varied from minimum of 1 band for the primer (Bmac0067) to maximum 3 bands for the primers (Bmag0125, Bmag 0225) in an average of 2 bands for each primer.

Cluster analysis and Dendrogram showed the highest PDV (81.82) between untreated plants of Fourat 1 and Gamma treated ones, while it was the lowest (45.45( between treated and untreated plants , which indicates wide genetic diversity among them.