تأثير درجة الحرارة في الفعالية و المقاييس الثرمودانياميكية لأنزيمات الفوسفاتيز في بعض ترب جنوبي العراق

الملخص EN

The objective of this paper is to study the activity of the phosphatase enzymes which included acid , alkaline phosphatase and Inorganic pyrophosphatase enzymes in different locations in South of Iraq .This study included two depths ( 0----15 cm and 15- 30 cm ) This study focused on the effect of temperature difference from 10 ---- 80°C on enzyme activity .As well thermodynamics measurements were determined for acid , alkaline phosphatase and Inorganic pyrophosphatase enzymes included activity energy ( Ea ) and temperature coefficient(Q 10 ).

The increasing of temperature from ( 10 – 60 °C) leads to increasing the activity of alkaline and acidic phosphatase enzymes and Inorganic pyrophosphatase in all soils and for two depths where the activity of enzymes on depth ( 0 -- 15 cm ) and ( 15 – 30 cm ) in the following order acidic > Alkaline > Inorganic pyrophosphatase In contrast the increasing of temperature from ( 60 – 80 °C) leads to reducing the activity of all enzymes for the most soils and for two depths .As well the increasing of temperature leads to difference in thermodynamics measurements for enzymes where they reduced the value ( Q10 ) with the increasing of the reaction temperature .

Different values of Ea appeared for enzymes with the difference of soils under study and with the difference of depths depending on the difference of enzyme concentration and soil properties.