دور بيكاربونات الصوديوم و بكتيريا Bacillus polymyx و المعاملة الحرارية و التداخل فيما بينهم في تثبيط نمو الفطر Aspergillus parasiticus المرافق لنمو ثمار التفاح المستورد و المحلي المشخص جرئيا و المنتج باسم أفلاتوكسين B1

الملخص EN

This study was conducted to isolate and diagnose the fungus Aspergillus parasiticus Combined local and imported apple fruits using traditional methods have confirmatory diagnosis of this fungus was conducted using the Polymerase Chain Reaction technique (PCR) and test the effectiveness of a number of integrated pest management programs to control it.

chemical detection using thin layer chromatography plates Technology (TLC) showed that seven isolates of the fungus A.parasiticus from 12 isolation (58%) Would producing poison aflatoxin B1.

Insulation results also showed the presence of two isolate of bacteria ,have the ability to contrast with fungus A.parasiticus and gave the isolation of bacteria Bp4Bacillus polymyxa isolated from apple fruits inhibition highest percentage inhibition of the fungus as reached at 80%, and the results also showed that the integrated pest management program, consisting of isolation Bp4 with substance sodium bicarbonate is the best in terms of the ability to fight against rot caused by the fungus A.parasiticus, as the percentage of infected fungus A.parasiticus of 8.53% which was significantly differente from the increase in the control treatment (fruits treatment vaccine A.parasiticus only), which was to 58.33%, while the infection rate of the injured fruits that applied on it the same program 75% at the time that infection rate in the treatment of comparison reaghed 91.66.