اكتشاف وجود الشكل الممشوق ضمن بؤر الآفات في اللايشمانيا الجلدية عند المضيف البشري

الملخص EN

Leishmaniasis is an endemic parasitic disease in 98 countries.

It is widely distributed throughout the world, caused by vector-borne, obligate, intracellular hemoflagellates of the genus leishmania.

The parasite continues its life cycle transforming to promastigote in the midgut of the sandfly vector and is transmitted to the human host in the form of promastigote through the bite of the sandfly.

Other less encountered forms of transmission are because of a laboratory accident, direct person-to-person transmission, organ transplant and blood transfusion.

There is evidence that leishmaniasis may be transmitted either in utero or during the peripartum period.

The promastigote form is considered the primary organism of disease transmission between the vector and the host.

By not having a chance to continue its life cycle and transform into promastigote within the vector sandfly, and considering the many different routes of transmission other than the sandfly bites, it is reasonable to assume an alternative possible existence of the promastigote form of the parasite in the infected lesion of cutaneous leishmaniasis in human host.

The information presented below indicates that a real transformation of amastigote to promastigote form occurs within the human host cutaneous lesion in the extracellular fluid after the macrophage membrane eruption and the amastigote release.

New techniques are recommended for future studies to confirm these findings including real time Polymerase chain reaction (PCR) and applying the immunohistochemistry techniques using novel monoclonal antibody (mAb) against the parasite flagellate (promastigote form) cell wall component.