محاولة التمنيع باستخدام الليشمائية مثبطة الانقسام بMitomycin-c

الملخص EN

Leishmania tropica is a protozoan parasite that causes cutaneous disease in the old world.

The study aimed to determine cell-mediated immunity by measuring serum IFN-γ and IL-4 levels, in BALB/C mice immunized with 107 mitomycin-c treated live leishmania promastigotes (inhl), combined with 2µg IL-12, or immunized with 2µg IL-12 only(controls), or immunized with 107 wild type leishmania promastigotes.

We found that inhl induce significantly higher levels of IFN-γ 360.62 pg/ml than controls 4.36 pg/ml and then WT 41.72 pg/ml.

beside of this, inhl induce significantly lower IL-4 levels 3.18 pg/ml than WT 69.82 pg/ml and then controls (lower than 2pg/ml).

Our study revealed that inhl induce cellular mediated immunity comparable with controls and WT.