الكشف عن مدى تلوث ثمار طرز مختلفة من الفستق الحلبي بالأفلاتوكسينات بطريقة اليتانول-ماء باستخدام جهاز الروماتوغرافي السائل عالي الأداء

الملخص EN

The study was conducted in the General Commission for Scientific Agricultural Research at Sweida Research Center, to evaluate the possibility of pistachio contamination with aflatoxins.

Thirteen P.

vera genotypes (Ash.1, Ash.2, Ash.3, Ash.4, Ash.5, Ash.6, Bat.1, Bat.2, Ajami.1, .1, .1, .1, Ajami.2, Beadi.1, Turki.1, and Turki.2) in comparison with a commercial sample of grated P.

vera nuts were evaluated using the methanol-water method HPLC.

Results showed that 4 of 13 P.

vera genotypes were contaminated with aflatoxins, particularly the type B1 in the range of 0.0-9.06 μg/kg.

The main concentration was detected in Ash.5 genotype, in addition to the B2 type (1.634 μg/kg).

The contamination was related to the high sensitivity of some P.

vera genotypes to different insects and diseases, early splitting of nuts in some genotypes, in addition to the poor agricultural practices in the field.

Three types of aflatoxins were found in the commercial sample of grated nuts; B1, B2 and G1 at 9.48, 3.92 and 0.85 μg/kg, respectively, which were significantly different from all other genotypes at 99% confidence level.

Contamination level reached 30.77% after storage for three months when the nuts of the different genotypes were not surface sterilized, in addition to the contamination of the commercial sample.

The results endorsed the high efficiency of HPLC system in detecting aflatoxins contamination in pistachio nuts.