دراسة فسلجية نسجية لتأثير المستخلص الإيثانولي لأوراق الشاي الأخضر و الأسود في إناث الجرذان البيض المستحثة بالمضاد الحيوي الجنتامايسين

الملخص EN

The researching interests at the last period have been tend to the hepatotoxicity and nephrotoxicity were resulted from the effect of some drugs , antibiotic ,heavy metals , toxins, and other chemical materials which induce the oxidative stress and increase free radicals production such as :hydrogen peroxide and hydroxyl radicals, this leads to inactivate or inhibit the enzymatic and non-enzymatic defense systems of antioxidants in the liver ,also change the chemical structure of proteins ,nucleic acids and elevation in the effectiveness of liver enzymes, that considers an obvious evidence for the hepatotoxicity .In addition ,these harmful radicals present a shrinking in the mesangial cells of renal glomerulus and decrease in the glomerular filtration rate , therefore the current study was carried out ,and aimed to use some of the plant extracts of highly antioxidants content which may be revealed anti-hepatotoxicity and nephrotoxicity impacts.

The study was performed at the animal house of Biology department / College of Education for girls and including the use of 30 female laboratory rats of the Sprague-Dawley strain randomly divided into six equal groups :the first group was the negative control group administered with normal saline solution(0.9%) ,the second group was the positive control ( that treated with Gentamicin)at the dose of 80 mg/kg intraperitoneally , the third group was intraperitoneally injected with the Gentamicin 80 mg/kg + Green tea extract at the concentration of 300mg/kg orally, the forth group was received Gentamicin 80 mg/kg intraperitoneally+ Black tea extract at the concentration of 300 mg/kg orally ,the fifth group was orally given 300 mg/kg of Green tea extract , and finally the sixth group which orally subjected to the extract of Black tea at the concentration of 300 mg/kg .The treatments of all experimental animals were once daily for a period of seven days.

During the current study, the body weight of animals, as well as liver and kidney weights were recorded, also the estimation of some biochemical parameters including: the effectiveness of alanine amino transferase ( ALT) , aspartate amino transferase (AST) and alkaline phosphatase (ALP), the levels of creatinine, urea, glutathione and malondialdehyde, while the histological study comprising preparation of sections for the liver and kidney tissues.

The statistical analysis of the study results was showed :- a significant decline(P< 0.05) in the body weight at the positive control group in compared with the negative control and the two groups that only treated with Green and Black tea respectively, in contrast the injection with Gentamicin (positive control) caused significant increment (P< 0.05) in the weights of liver and kidney, the effectiveness of ALT,AST and ALP, levels of creatinine ,urea , and (MDA) as compared with the other groups of study .As regard with the (GSH) level, was significantly decreased(P< 0.05) at the positive control group in comparison to the other experimental groups.

The histological examination of female livers that treated with Gentamicin (positive control group ) pointed to obtain many of histopathological alterations including ,degeneration and necrosis in some of liver cells , hemorrhage and hypertrophy in others, dilatation in some of central veins of liver lobule , congestion , hemorrhage , infiltration of the inflammatory cells and gathered around some of central veins.

Concerning to the groups of Gentamicin+Green tea, Gentamicin+Black tea, as well as, the groups that only treated with Green and Black tea respectively, did not appear any pathological change in the liver tissue when compared with the negative control group.

In relation to the cortex and medulla sections of female kidneys that injected with the Gentamicin antibiotic, these sections were revealed degeneration ,necrosis, ,and hemorrhage in some of renal glomeruli ,as well as, atrophy ,or hypertrophy in others, in addition to, the tubular necrosis, edema and hemorrhage within the interstitial tissue of medulla .With regard to the other experimental groups, Gentamicin+Green tea ,Gentamicin +Black tea ,Green tea, and Black tea respectively, there was no histological alteration recorded in this study in comparison with the negative control group.

It was concluded from the current study that the ethanolic extract of Green and Black tea leaves was showed a preventive influence in the protection of hepatic and renal histological structures from the toxicity of the dose 80mg/kg of Gentamicin, this may be explained to their content of antioxidants which have highly efficiency for scavenging the free radicals and removing them outside the living cells, that leads to prevent or alleviate the damages were caused by the various types of reactive oxygen species that probably resulted from the injection with Gentamicin antibiotic ,also the findings of current study were conducted to, no significant differences between the efficacy of both types of tea in the ability to ameliorate the functional performance of liver and kidney, as well as reduce the regression of biochemical and oxidative stress indices that caused by the adverse effects of deliberated antibiotic.