التنسيل و التعبير البروتيني لمورثة بيبتيداز الإشارة (النمط I)‎ من السلمونيلا التيفية

الملخص EN

The lepB gene encoding type I signal peptidase enzyme was amplified by PCR using extracted Salmonella typhi genomic DNA as a template, A primer pair were designed from this gene containing restriction sites of BamHI and NdeI.

The gene and the expression vector pET15b were digested with the previous restriction enzymes, the gene was then inserted into the vector to construct the recombinant vector pET15b/lepB which was transformed into E.coli BL21 (DE3), this gene was expressed by IPTG induction.

The recombinant protein was purified with chromatoghraphy affinity.