تضخيم كل من المورثات tyv ﻭ prt ﻭ invA لتحديد النمط المصلي التيفي ﻭ نظير التيفي لعزلات سلمونيلا معزولة من عينات غذائية

الملخص EN

Typhoid and paratyphoid fever are two diseases responsible for the death of many people annually.

The responsible pathogens for these diseases are Salmonella typhi and Salmonella paratyphi respectively.

We should differentiate between these two human pathogenetic serovars and the other serovars which are less pathogenic for human, therefore we apply one of the molecular biology techniques to determine these two serovars in isolates obtained from food samples.

65 food samples including milk, cheese and eggs samples were collected from Damascus suburb.

These samples were cultured on differential media to detect the samples which contain salmonella.spp, then morphological and biochemical characterizes were studied, after that Salmonella typhi and Salmonella paratyphi were determined by Multiplex PCR using three primer pairs which were designed to amplify tyv, prt and invA genes.

Finally 47 Salmoenella spp.

isolated were obtained (72.3% of the samples), contained: 16 Salmonella typhi isolates (34.04% of the Salmonella spp.

isolates), 13 Salmonella paratyphi isolates (27.65% of the Salmonella spp.

isolates), 18 Salmonella spp.

isolates which belong to the other Salmonella serovars (38.29% of the Salmonella spp.

isolates).