تحضير اللقاح المبطل الزيتي لفايروس نيوكاسل و استخدامه في تقنية تلقيح الأجنة

الملخص EN

In this study ,eighty(80)fertile eggs taken from commercial breeders were used .These eggs were divided into(2)equal group,group (1) was vaccinated with prepaired oil inactivated vaccine &the second group as control group which given normal saline.Result show that can be used the oil inactivated vaccine to protect the chick embryo,which did not reveal any negative effect on the hatchbility & livability percentage.Heamagglutination Inhibition test(HI)&ELISA test were used,the result revealed increase of inhibitor antibodyes in group (1) with level of P<0.05 in comparative with group(2) at the age of 1,7,14 days after hatching.Early challenge test was used in 14 day after hatch to evaluate this vaccine the result show resistance of group (1) to challenge dose with virulent Newcastle virus & protective percent reach %93.33 compairtive with control group which give %26.66.