استعمال تقنية تفاعل متسلسل البلمرة PCR للكشف عن جين copA المسؤول عن مقاومة النحاس في جرثومة العصيات الرقيقة Bacillus subtilis

الملخص EN

High concentrations of heavy metals result toxic effected on enzymes, functional groups and destroyed of membranes.

Some of bacteria apple to converted of heavy metals from harmful to un harmful forms .

Bacillus subtilis was presence in contaminated environment due to responses and accumulation of heavy metals such as copper because of possession of copA gene is code to proteins accumulation of heavy metals from surrounding environmental .

In the present study for isolation of DNA and detection of copA gene of molecular weight 258pb