التعبير الجيني و وفرة نسخ mRNA لجينات نقل عناصر الطاقة داخل الأمعاء الدقيقة لفروج اللحم عند التغذية على نسب مختلفة من الطاقة و البروتين

الملخص EN

This study was conducted in both Poultry Farm/Animal Resources Department/ College of Agriculture / University of Basra and laboratories of Molecular Biology/ London/ United Kingdom (The Molecular Oncology Unit Laboratories of Guy's and St Thomas's Hospital London / UK).

Molecular analysis of the samples and the detection of a number of gene expression of several feed transporter genes in the small intestine of broiler chickens when fed diets with different levels of energy and protein.

A total of 250 unsexed one day old broiler chicks (Ross breed) were randomly distributed on five experimental treatments each with three replicates of 15 chicks.

The first treatment was control.

The second treatment and the third treatment consisted high and low protein respectively.

While the fourth the fifth treatment consisted high and low Energy respectively.

The results revealed the followings: Females fed 20 % cp and 3199 kcal/kg ME diets reordered highest SGLT1 gene expression (4.70ng) within their ileum.

Males fed 20 % cp and 3199 kcal/kg ME diets reordered highest GLUT5 gene expression (6.37 ng) within their ileum.

All genes associated positively and significantly with body weight, growth rate, carcass cuts percentages (legs, breast, intestine length and weight) at the age of 35 days and negatively with feed consumption and feed conversion ratio.

Saccharide transporter genes abundance improves with the increase of ME level in the diets.

Male gave higher mRNA copies for GLUT5 in comparison with females, While SGLT1 was increased in females.

It is possible to manipulate nutrient transporter gene expression by feeding.

That may reflect on birds performance.