تشخيص فايروس موزائيك الخيار CMV على الفلفل باستخدام النباتات الكاشفة و الأشرطة المناعية و تقنية تفاعل البلمرة التسلسلي العكسي (RT- PCR)‎

الملخص EN

This study was conducted to identify cucumber mosaic virus (CMV) on pepper plants and determine the host range and the source of primary infection, by using indicator plants, immune strip test and PCR.

The infection was done by using the sap.

of pepper plants containing CMV upon of some indicator plants like cucumber (Cucumis sativus L),tomato(Lycopersicon esculentum Mill) , zucchini ( Cucurbita pepo L) , Pumpkin (Cucurbita maxima L) , eggplant (Solanum melongena L.) cowpea (Vigna unguiculata ) tobacco (Nicotiana tabacum cv.samsun , Nicotiana rustica )and Wall goosefoot(Chenopodium murale L.) The results showed that all above-mentioned plants were infected with CMV .The symptoms of infection were mosaic , Malformation and stunting in cucumber ,while tomato leaves changed to filiform shape.

The symptoms of zucchini , Pumpkin and eggplant were in the form of systemic mosaic and stunting of egg plants , while the symptoms upon cowpea plants were pale local lesion leaves .and in the Wall goosefoot plants the symptoms were unorganized spots.

The two varieties of tobacco showed vein clearing.

Symptom.

The test of immune strip showed the existence of CMV in cucumber, tomato , pepper and cowpea plants by clear precipitation line compare to control plants.

The diagnosis of CMV virus was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR).

The plant specimens collected from areas under study were gave positive results, the RT-PCR reaction with primer CMV1 and CMV2 for CMV-coat protein gene (CMV-cp Gene) yielded 486-488 base pair DNA bands while the specimens from non- infected plants amplified no bands.

These results indicated that the CMV virus detected in present study belong to subgroup I.