التنوع الوراثي لحشرة حافرة أوراق البندورة الطماطم باستخدام تقنية التضخيم العشوائي المتعدد الأشكال للحمض النووي

الملخص EN

Tomato leaf miner Tuta absoluta (Meyrick) is an important invasive insect, which entered Syria lately and became established.

The aim of the present study is to investigate the genetic diversity of tomato leaf miner in two regions; Efrine and Al-Sfireh.

Genomic DNA was extracted from one larva per sample using CTAB method with minor modifications.

Extracted DNA concentration, purity and integrity was measured.

Random amplified polymorphic DNA (RAPD) technique was used to study the genetic diversity of tomato leaf miner using 24 random primers in polymerase chain reaction (PCR).

PCR products were separated in 1.5% agarose gel to assess the similarity between the samples.

Polymorphic DNA fragments were detected by 5 RAPD primers out of 24 tested.

Based on the polymorphic information content (PIC), the best results were obtained from the marker OPS-11 with 0.44 calculated value.

PCR–RAPD analysis showed high genetic diversi-ty; the similarity ranged between 0.10 and 0.91, but it was less than 0.40 for the majority of samples.

Unique locus with 300 bp size was detected using OPP-16 marker in the insect samples collected from the green house.

Based on regions or locations from where the leaf min-ers were collected, insect genotypes were distributed in four clusters: I, II, III and IV with similarity coefficient of 0.42.

Clusters obtained were not related to the plant part from which the insects were collected from.