Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City
العناوين الأخرى
في بعض المكورات العنقودية السالبة لأنزيم التجلط (tet K ,tet M) التحري عن جينات المقاومة لمتراسايكلين المعزولة من مصادر سريرية مختلفة في مدينة أربيل
المؤلفون المشاركون
Barzani, Khadijah Khalil
Abd Allah, Zayn Abd al-Ilah
Hydaeat, Soma P.
Muhammad, Asya Sadiq
المصدر
Journal of University of Anbar for Pure Science
العدد
المجلد 10، العدد 2 (31 أغسطس/آب 2016)، ص ص. 11-20، 10ص.
الناشر
تاريخ النشر
2016-08-31
دولة النشر
العراق
عدد الصفحات
10
التخصصات الرئيسية
العلوم الاقتصادية والمالية وإدارة الأعمال
الملخص EN
Thirty two isolates of coagulase negative staphylococci was isolated and identified from clinical sources ; including eight isolates (12.5%) of each of Staphylococcus hominis and Staphylococcus haemolyticus from (64) patients urine which suffering from urinary tract infection and eight isolates (16%) of each of Staphylococcus epidermidis and Staphylococcus auricularis from (50) ear patients which suffering from otitis in Rizgary teaching hospital in Erbil city were isolated depending on Vitek 2 compact system .
The susceptibility tests by using disc diffusion agar method were determined for all isolated coagulase negative Staphylococci to 10 different antibiotics .
From obtained results it was observed that all isolates were 100% sensitive to ceftriaxone and novobiocin, while higher resistance was obtained to tetracycline with percentage 50% and they showed differnt resistant to other remaining antibiotics.
On the other hand, all isolated Staphylococcus hominis, Staphylococcus haemolyticus , Staphylococcus epidermidis and Staphylococcus auricularis isolates were tested for tetracycline resistant genes tetK and tetM by using PCR and the results showed that 75% of Staphylococcus hominis, Staphylococcus haemolyticus and Staphylococcus epidermidis were carried tetM gene with product size about 158 bp while 50% of Staphylococcus auricularis was carried the above mentioned gen .
Also it was found that 50% of Staphylococcus hominis and Staphylococcus haemolyticus isolates in this study were harboring tetK gene with product size 360 bp and no tetK gene was observed in Staphylococcus epidermidis and Staphylococcus auricularis .
Also the antibacterial effect of two local species of menthes against resistant isolated bacteria by using the Minimum Inhibitory Concentration (MIC) method were studied .
The results showed that the MIC for aqueous extract for both plants Mentha longifolia and Mentha piperita were 1600μg/ml and it were 1400μg/ml for alcoholic extract of both plants against most Staphylococcus speciese except Staphylococcus epidermidis which the MIC was 1200 μg/ml for aqueous and 1000μg/ml for alcoholic extract of Mentha piperita, while it was 1200μg/ml for both the aqueous and alcoholic extracts for Mentha longifolia
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Barzani, Khadijah Khalil& Abd Allah, Zayn Abd al-Ilah& Hydaeat, Soma P.& Muhammad, Asya Sadiq. 2016. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science،Vol. 10, no. 2, pp.11-20.
https://search.emarefa.net/detail/BIM-816178
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Barzani, Khadijah Khalil…[et al.]. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science Vol. 10, no. 2 (2016), pp.11-20.
https://search.emarefa.net/detail/BIM-816178
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Barzani, Khadijah Khalil& Abd Allah, Zayn Abd al-Ilah& Hydaeat, Soma P.& Muhammad, Asya Sadiq. Detection of tetracycline resistant gen (tet K, tet M ) in some coagulase negative staphylococci isolated from different clinical sources in Erbil City. Journal of University of Anbar for Pure Science. 2016. Vol. 10, no. 2, pp.11-20.
https://search.emarefa.net/detail/BIM-816178
نوع البيانات
مقالات
لغة النص
الإنجليزية
رقم السجل
BIM-816178
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر