Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron)
المؤلفون المشاركون
Mehraj, Sayyid Sana
Kamili, Azra N.
Nadhir, Ruqayah
Haq, Ihtisham
Balkhi, Henah Mehraj
المصدر
Saudi Journal of Biological Sciences
العدد
المجلد 25، العدد 8 (31 ديسمبر/كانون الأول 2018)، ص ص. 1603-1608، 6ص.
الناشر
تاريخ النشر
2018-12-31
دولة النشر
السعودية
عدد الصفحات
6
التخصصات الرئيسية
الموضوعات
الملخص EN
Broadly speaking proteomic studies are one of the various techniques of utmost importance for understanding complex biological processes that occur under inductive conditions and revealing the multidimensional aspects of Crocus sativus in biological systems.
In order to get an insight into the molecular changes and to characterize the variations in protein expression of C.
sativus, a detailed proteomic analysis on one-dimensional gel electrophoresis is one of the basic steps to accomplish.
We have compared total protein profiles of C.
sativus extracted by three different recipes and analyzed on 10% sodium dodecyl sulfate polyacrylamide gels.
Gels were subjected to densitometric analysis for further characterization.
Among three different protocols NP-40 extraction buffer recipe resulted in the extraction of proteins most efficiently with minimum background and streaking.
There was maximum solubilization of proteins with high efficiency.
Such a profile can be used for high precision analysis of differential protein expression.
This work is an attempt to assist researchers in effective extraction of proteins from C.
sativus.
As a researcher faces a perplexing array of choices as where to start we describe a method based on our collective analysis of the different protein protocols.
This paper presents a method that could be applied at the outset of any proteomic study.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kamili, Azra N.& Nadhir, Ruqayah& Haq, Ihtisham& Balkhi, Henah Mehraj& Mehraj, Sayyid Sana. 2018. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences،Vol. 25, no. 8, pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Mehraj, Sayyid Sana…[et al.]. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences Vol. 25, no. 8 (Dec. 2018), pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kamili, Azra N.& Nadhir, Ruqayah& Haq, Ihtisham& Balkhi, Henah Mehraj& Mehraj, Sayyid Sana. Comparative evaluation of extraction methods for total proteins from crocus sativus L. (Saffron). Saudi Journal of Biological Sciences. 2018. Vol. 25, no. 8, pp.1603-1608.
https://search.emarefa.net/detail/BIM-832877
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references : p. 1608
رقم السجل
BIM-832877
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر