التباينات الأليلية لمورثات الديهيدرين في بعض الطرز الوراثية من القمح المنزرع (القاسي و الطري)‎ و البري

الملخص EN

This study was conducted at the Biotechnology Lab.

- Faculty of Agriculture – Damascus University (Syria) in 2016.

The aim of the research was to detect the allelic variations of Dehydrin genes in different durum and bread wheat genotypes: horani, juri, sham6, sham8, doma4.

doma2, sham3, sham9, doma3), and three wild types (Aegilops ovata, Ae.

triuncialis, Ae.

geneculata).

Results of Dehydrin gene variation (responsible for drought tolerance) showed significant differences among the studied genotypes.

Variation in the molecular weight between loci per gene was very high in some cases, while the similarity in other cases was at a high degree, and it was easily distinguishable by 4% agarose gel.

The PCR results of the Dehydrin gene Dhn12 showed a monomorphic pattern in the four genotypes: sham6, sham8, doma4, and doma2.

While Dhn6 gave two patterns emerged in the 6 genotypes: sham8, sham9, doma4, sham3, horani, and Ae.

ovata.

It is noticeable that both genotypes juri, and doma3, and the wild species Ae.

triuncialis, Ae.

geneculata had only one monomorphic pattern.

There were three patterns (A, B, C) of Dhn4 and Dhn3 varied in the studied genotypes, three of four patterns of the Dehedrin gene Dhn7, appeared in doma3.

The PCR reaction also showed five patterns for the Dehedrin genes: Dhn9, Dhn16, Dhn15, and six patterns for two dehedrin genes: Dhn5 and Dhn13.

The results showed that the dehydrine gene Dhn9 had the largest number of polymorphic patterns, with 30 patterns in all studied genotypes, followed by the gene Dhn15 with 24 patterns, while the gene Dhn12 gave the lowest number of patterns (only 4) in the studied genotypes.

While, the genotype (doma4) was superior in the number of patterns which gave 19 genetic patterns, while doma3 gave the lowest number of patterns (10 patterns).