الكشف عن الفيتوبلازما المسببة لمرض البرعم الكبير على البندورة الطماطم في سورية

الملخص EN

Since 2013, symptoms similar to those produced by the phytoplasma disease "Big bud" were observed on tomatoes (Solanum lycopersicum L.) grown in the field in east of Homs city, Syria.

Diseased plants were characterized by twisting, corrugated, yellowing or reddening of leaves.

The sepals of the flowers acquired hypertrophied form, were fused together and created a bell-shaped sterile bud (phyllody) of green or anthocyanin color.

The stems of the plants were lignified, and phloem necrosis was observed on the stem.

In mid-September 2017, samples of 12 diseased and 2 healthy (control) tomato plants were collected from the Syrian province of Homs in the villages: Al-Nozha, Al-Diba, and Al-Aliyat (30Km east of the city of Homs).

Phytoplasmas were dectected in 11 diseased samples.

RFLP analysis with restriction enzymes AluI, HhaI, MseI (Tru1I), Sau3AI, and TaqI showed that they all correspond to phytoplasmas of the Clover proliferation subgroup 16SrVI-A, or ‘Candidatus phytoplasma trifolii’ species.